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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Blood Cells Mol Dis. 2009 Oct 27;44(1):7. doi: 10.1016/j.bcmd.2009.10.002

Fig. 2. cJun and CREB1 phosphorylation levels increase after drug treatment.

Fig. 2

Protein extracts were isolated from K562 cells treated with NaB (2 mM) or TSA (0.5 μM) for 48 hrs (see Materials). A) Shown is the western blot performed with phosphorylated-cJun (p-cJun) and total-cJun (t-cJun) antibody. B) Western blot analysis results for p-CREB1 and t-CREB1 antibody is shown. C) Quantitative data obtained from the western blot gels using ChemiDoc software (Biorad) are shown in the graphs. Shown are p-CREB1/t-CREB1 (black bars) and p-cJun/t-cJun (gray bars) protein levels in the absence (-) or presence (+) of drug treatments. Data are shown as the mean ± SEM, *p<0.05 was considered significant.