Figure 1. Schematic of the flow system set-up.
The Streamer device consists of a parallel-plate laminar flow chamber accommodating six 75 × 25 × 1 mm slides. This is a closed system in which the medium is re-circulated. All components of the system, except the pump, are autoclaved. A MasterFlex Pump circulates the medium through sterile MasterFlex silicone tubings (L/S 17). The pulse dampeners trap and remove air in the system.