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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: J Proteomics Bioinform. 2009 Jan 1;2:445–454. doi: 10.4172/jpb.1000104

Figure 2. Quantitative proteomic analysis of vascular endothelial cells in high glucose.

Figure 2

Bovine aortic endothelial cells (BAEC) were cultured in normal (5 mM, NG) or high (22 mM, HG) glucose. Cells were either shear stressed or kept in stationary conditions (no flow) as control. Membrane fractions were prepared from the shear stressed and control cells. The membrane associated proteins were digested with trypsin, and the peptides were labeled with iTRAQ reagents. Labeled peptides were combined, fractionated by strong cation exchange chromatography, and analyzed by LC-MS/MS.