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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: J Proteomics Bioinform. 2009 Jan 1;2:445–454. doi: 10.4172/jpb.1000104

Figure 6. Western blot analysis of ubiquitinated proteins in BAEC.

Figure 6

Immunoblot analysis of ubiquitinated membrane associated proteins in cells cultured in NG and HG under control conditions and after 3 hours of shear stress. Actin served as the loading control. The results showed that shear stressed cells cultured in NG and in HG had increased level of ubiquitination in a broad region of membrane proteins shown as enhanced smears. Bar graph are expressed as the means ± SE of three independent experiments by densitometric analysis of immunoblots normalized to actin using UN-SCAN-IT gel 6.1 (*p<0.05). SS: shear stress.