Fig. 1.
Normalization reduces overall variance of CT values. This mean-centering adjustment was performed to remove potential RNA loading differences across samples. For each gene, CTvariance across all studied samples was calculated for each of the 3 technical replicates before (★) and after (○) normalization (each point represents a single replicate of data from 7 mildly and 7 severely affected kidneys). The normalization reduced overall variance of CT values for all genes, although at different rates for specific genes.