Table 2.
XenoMouse®-derived human anti-α3(IV)NC1 mAb
| mAb |
VH (gene) |
CDR1 |
CDR2 |
CDR3 |
JH |
|---|---|---|---|---|---|
| Heavy chain analysis demonstrating multiple VH genes may be used to encode pathogenic antibodies | |||||
| F1.1 | VH4 (DP-70) | Δ 3 bp | Δ 3 bp | NI | Δ 5b (6 bp) |
| F2.1 | VH3 (DP-50) | Δ 14 bp | germline | NI | NI |
| F3.1 |
VH3 (DP-47) |
germline |
germline |
Δ D3-22 (6 bp) |
Δ 6b (germline) |
| Light chain analysis demonstrating multiple VK genes may be used to encode pathogenic antibodies | |||||
| F1.1 | VK2 (DPk-12) | Δ 2 bp | germline | germline | Δ 5 |
| F2.1 | VK3 (DPk-22) | germline | germline | Δ 1 bp | Δ 2 (3 bp) |
| F3.1 | VK3 (DPk-21) | germline | germline | germline | Δ 1 (2 bp) |
Blast search alignment for F1.1 was closest to Macaca fasicu-laris Ab derived from a phage display library vs. hepatitis A (not shown), but the others were not. Δ = Change from germline; VH = variable heavy chain; CDR 1, 2 and 3 = complement determining regions 1, 2 and 3; JH = junction heavy variable to heavy constant region; NI = not identifiable; bp = base pairs.