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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Int J Cancer. 2010 Apr 1;126(7):1666. doi: 10.1002/ijc.24961

Fig. 4. Abrogation of the NKT cell-IL-13-IL-4R-STAT-6 immunoregulatory pathway does not enhance vaccine efficacy.

Fig. 4

1×105 irradiated (25,000 rad) CT26 cells were injected sc. into wt. BALB/c, BALB-CD1KO (A), BALB- IL-13 KO (B), BALB-IL-4-13KO (C), BALB-STAT-6 KO (D), BALB-IL-4R KO (E), or BALB-IL-4 KO (F) mice. 3 weeks after vaccination, 1×106 live CT26 cells were injected sc. into these mice. Tumors were measured by a caliper gauge, and tumor size was determined as the product of tumor length (mm) × tumor width (mm). Five female BALB/c mice were used for each group per each experiment. Mice were euthanized when the tumor area exceeded 1 cm2, and data were represented as percent survival. The results were pooled from two independent experiments. p<0.001 in A and B, p<0.002 in C, p<0.0005 in D, E, and F by log-rank test between un-vaccinated BALB/c mice and vaccinated BALB/c mice. p<0.01 in A by log-rank test between un-vaccinated BALB/c mice and CD1KO mice. p<0.02 in E by log-rank test between un-vaccinated IL-4R KO mice and vaccinated IL-4R KO mice.