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. 2009 Oct 7;29(40):12497–12505. doi: 10.1523/JNEUROSCI.3892-09.2009

Figure 3.

Figure 3.

Prx2 is phosphorylated by cdk5 in global ischemia and leads to neuronal death. a, Quantification of surviving CA1 neurons expressing GFP (n = 5), Wt (n = 11), as well as T89A (n = 10) or T89E (n = 6) forms of Prx2 after 4VO. AAV-mediated expression of WtPrx2, Prx2T89A, Prx2T89E in hippocampus was detected by Western blot analysis using anti-Flag antibody. b, Analysis of pPrx2 level in hippocampus at the time points after 4VO by Western blot. The membrane is representative of n = 6 experiments and graph presents densitometry values of pPrx2 relative to Prx2. c, Inhibition of cdk5 attenuates pPrx2. Rats were unilaterally injected with AAV expressing DNcdk5 into hippocampus and subjected to 4VO. Total proteins from both sides of the hippocampus were analyzed for pPrx2 by Western blot at 24 h after 4VO. Expression of DNcdk5 has been shown using anti-Flag antibody. The membrane is representative of n = 3 experiments and graph presents densitometry values of pPrx2 relative to Prx2. ipsi, Virus-injected side; cont, noninjected side. “NC” represents nonstroked control animals. Data are presented as mean ± SEM (*Student's t-test, p < 0.05).