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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2009 Nov 5;48(2):306. doi: 10.1016/j.freeradbiomed.2009.11.001

Figure 3.

Figure 3

Subcellular localization of DAF-NO adduct following NO delivery. DAF-loaded RPMVECs exposed to DeaNONOate (5 μM) were stained with (A) Lysotracker Red and (B) brefeldin A- BODIPY 558/568 conjugate to simultaneously assess DAF-NO adduct localization in the lysosomes and golgi apparatus, respectively. (C) RPMVECs transfected with mitochondrially-targeted DsRed (Mito-DsRed) were also loaded with DAF to assess DAF-NO adduct localization in the mitochondria following DeaNONOate application.