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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2009 Nov 10;43(2):188. doi: 10.1016/j.mcn.2009.10.009

Figure 3. Colocalization of SPAR and PDLIM5 in COS-7 cells.

Figure 3

(A-C) COS-7 cells were singly transfected for SPAR, PDLIM5a, or SPAR ΔAct2 as indicated and immunostained for the exogenous protein. Arrows in (B) indicate examples of PDLIM5a enrichment at apparent membrane ruffles. (D) COS-7 cells doubly transfected with SPAR and PDLIM5a, and immunostained for SPAR (D1) or PDLIM5a (D2), with merged image shown in D3. Arrowheads, SPAR clusters that recruit PDLIM5. Arrow, example of PDLIM5 enrichment at membrane ruffle that does not recruit SPAR. (E) COS-7 cells doubly transfected with PDLIM5a and SPAR ΔAct2 and immunostained for SPAR (E1) or PDLIM5a (E2), with merged image shown in E3. (F) COS-7 cells doubly transfected with PDLIM5a and Act2 domain and immunostained for Act2 (F1) or PDLIM5a (F2), with merged image in F3. (G) COS-7 cells doubly transfected with PDLIM5b and SPAR and immunostained for SPAR (G1) or PDLIM5b (G2), with merged image in G3. Scale bar in (A), 5 μm.