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. Author manuscript; available in PMC: 2011 Jan 3.
Published in final edited form as: J Proteomics. 2009 Nov 13;73(3):619. doi: 10.1016/j.jprot.2009.11.003

Fig. 1.

Fig. 1

Experimental design for in vivo proteomic analysis of −/+ HU sickle RBC membranes-RBC membrane samples (100 μg each) obtained from reticulocyte free, mature RBCs of five SS subjects not on HU were pooled and labeled with Cy3 fluorophore. This pooled control was compared against individual Cy 5 labeled sickle RBC membrane samples (100μg) obtained from reticulocyte free, mature RBCs of five SS patients on HU therapy.