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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2009 Oct 19;261(1):29. doi: 10.1016/j.cellimm.2009.10.008

Figure 1. Serum starvation, PMA and PMA/IM promote thymocyte apoptosis.

Figure 1

Apoptosis of thymocytes was determined after PMA, PMA+ ionomycin and serum starvation treatment. Cells were kept in the presence of PMA (1μM) with or without ionomycin (0.5μM) for 4 hours and serum starved for 24 hours. After the different treatments cells were stained with Annexin V-FITC and propidium iodide following the manufactures instructions and analyzed by flow cytometry on a BD LSR II or FACSAria using BD FACS Diva software (BD Bioscience). Data represent one of five different experiments with similar results. Cells exposed to vehicle (DMSO) did not differ from untreated cells.