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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Immunol. 2009 Oct 19;261(1):29. doi: 10.1016/j.cellimm.2009.10.008

Figure 6. Phosphorylation of BimEL by ERK activated by PMA treatment but not by s/s.

Figure 6

(a) Thymocytes were treated with PMA (1μM) for 30 minutes or serum-starved for 24 hours. Serum-starved cells were left untreated or stimulated with serum. (b) A one hour pretreatment with UO126 (10 μM) prevented the PMA-induced mobility shift of BimEL indicative of its phosphorylation. Cell lysates were resolved by SDS-PAGE and immunoblotted with the indicated antibodies. Actin was used for control equal loading of protein. The representative blots from 3 experiments are shown in each (a) and (b).