Figure 2.
Molecular outlines from interference-free NBD1 and NBD2. (A) Superimposed experimental SAXS curves from refolded NBD1 and NBD2. Inset: Guinier plots reflect homogeneous and monodisperse NBD1 and NBD2 in solution. (B) Distance distribution function, (P(r)), computed from experimental data and normalized to the maximum value of unity, demonstrated a maximum molecular dimension (Dmax) at 170 and 180 Å for NBD1 and NBD2, respectively. (C) Atomic force microscopy in contact mode mapped the topography of NBD2 molecules (186±18 Å, n=8).