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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Bioorg Med Chem Lett. 2009 Dec 3;20(2):700. doi: 10.1016/j.bmcl.2009.11.065

Figure 2.

Figure 2

FGFR-expressing Sum52 cells cultured in 24-well plates was incubated with different concentrations of purified FGF-1 containing 32 pM 125I-FGF. Following incubation for 1 hour at 4°C, the cells were washed 4 times with phosphate-buffered saline and extracted with 0.5% Triton X-100, and the radioactivity was measured in a γ-counter.