Table 1.
High-throughput screen of 201,368 compounds of the MLSCN library
| Screening Step | MLSCN library compounds |
|---|---|
| Mixture screen (4 compounds per well) | |
| Unique compounds tested | 201,3681 |
| Wells that showed >30% inhibition | 2,3282 |
| Initial hit rate3 | 1.2% |
| Retest (single compound per well) | |
| Compounds retested4 | 5266 |
| Number of compounds that showed >30% inhibition | 470 |
| Number of compounds that showed <30% inhibition | 4796 |
| Retest hit rate5 | 8.93% |
| Dose Response | |
| Compounds tested | 388 |
| Active compounds | 372 |
| Hits reclassified as inactive due to fluorescence increase in absence of enzyme | 358 |
| True hits | 14 |
| True hit rate6 | 0.007% |
1
64,020 compounds were tested twice and thus 265,388 compounds (including duplicates) were tested in total. See experimental procedures for details.
2
It is most likely that only one of the 4 compounds in each well was active.
3
Expressed as (2328/201,368)*100.
4
Active wells had 4 compounds (with minor exceptions) but not all compounds were available.
5
(470/5266)*100.
6
(14/201,368)*100