Figure 4.
Auts2 mRNA and protein expression in the developing hippocampus. A–B: At P0, Auts2 expression, as detected by ISH (gray or green), was apparent throughout the developing hippocampus where it colocalized with Tbr1 immunofluorescence (red). C–H: Auts2 protein, detected by immunofluorescence at P21, was apparent throughout the granule cell layer (GCL) of the dentate gyrus. Coexpression with specific markers of progenitor cells and differentiating neurons was assessed by double immunofluorescent labeling with C) Sox2; D) PCNA; E) NeuroD1; F) calbindin (CalB); G–G”) DCX; H–H”) calretinin (CalR). Scale bars: A 100 µm (applies to A, B); C: 50 µm (applies to C–F) G: 50 µm (applies to G–H”).