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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Gene Expr Patterns. 2009 Dec 3;10(1):9. doi: 10.1016/j.gep.2009.11.005

Figure 5.

Figure 5

Expression of Auts2 mRNA in brain regions outside the cortex at different stages of development. A–a) Double labeling for Auts2 mRNA revealed by in situ hybridization (green) and Tbr1 protein by immunofluorescence (red) in the E14 cerebellum. NTZ: nuclear transitory zone; EGL: external granular layer; CTZ: cortical transitory zone; RL: rhombic lip; VZ: ventricular zone. B) Postnatal day (P) 21 cerebellum labeled with Auts2 (green) in relationship to Tbr1 (red); b) expression of Auts2 protein (green, immunofluorescence) in Purkinje cells identified by calbindin immunofluorescence (red). C) Auts2 mRNA (green) was expressed in the dorsal thalamus (DT), in a complementary fashion compared to the eminentia thalami (ET), stained by Tbr1 (red, immunofluorescence). D) Auts2 mRNA (green) was localized in the P21 olfactory bulb mitral cells (MC) and the periglomerular cells (PGC); overlap with Tbr1 (red) was found in some MC. Also, some overlap of Auts2 mRNA (green) with GAD67 (red, d), a marker of GABAergic neurons, was detected in the GC (arrows). E) Strong coexpression of Auts2 protein (green) and TH (red) was found in the substantia nigra (SN) and the ventral tegmental area (VTA). Scale bars: A: 100 µm; a: 50 µm; B: 100 µm; b: 50 µm; inset b: 20 µm; C: 200 µm; D: 100 µm; d: 30 µm; E: 100 µm; inset E: 20 µm. Ages: A–a: E14, B–b: P21, C: E14, D–d: P21, E: P21.

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