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. Author manuscript; available in PMC: 2011 Feb 1.

Published in final edited form as: Biochim Biophys Acta. 2009 Nov 13;1802(2):269. doi: 10.1016/j.bbadis.2009.11.002

Chronic synthetic lethality complementation assay. Fresh overnight cultures of EWU5 transformed with plasmids bearing constitutively expressed wild-type ITPase (WT), P32T ITPase (P32T) or empty vector (EV) were serially diluted in LB media and 5 μl of each dilution was spotted in duplicate onto LB-ampicillin plates pre-warmed at 30 or 42°C. Plates were incubated at appropriate temperature for 48 hours and photographed under white light. (A) 30°C plate; (B) 42°C plate.

Chronic synthetic lethality complementation assay. Fresh overnight cultures of EWU5 transformed with plasmids bearing constitutively expressed wild-type ITPase (WT), P32T ITPase (P32T) or empty vector (EV) were serially diluted in LB media and 5 μl of each dilution was spotted in duplicate onto LB-ampicillin plates pre-warmed at 30 or 42°C. Plates were incubated at appropriate temperature for 48 hours and photographed under white light. (A) 30°C plate; (B) 42°C plate.