Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Nov 13;1802(2):269. doi: 10.1016/j.bbadis.2009.11.002

Figure 4.

Figure 4

Western blot analysis. EWU5 cells were transformed with plasmids bearing constitutively expressed wild-type ITPase (WT), P32T ITPase (P32T) or empty vector (EV) and grown at 30° and 42°C. Cells were harvested and total protein concentration was determined. Western blotting was performed as described in Materials and Methods with 5 μg of total protein from each cleared cell lysate using Rabbit PolyAb Anti-ITPA and HRP-conjugated secondary antibody. Lane 1, EV at 30°C; lane 2, WT at 30°C; lane 3, P32T at 30°C; lane 4, WT at 42°C; lane 5, P32T at 42°C.