Figure 5.
SK2009 did not inhibit the direct binding of NF-κB to DNA. Nuclear extracts (NE) were prepared from untreated KBM-5 cells or cells treated with 0.1 nM TNF for 30 min, incubated for 30 min with the indicated concentrations of SK2009, and assayed for DNA binding by EMSA. Data is the average of 3 independent experiments.