Skip to main content
. Author manuscript; available in PMC: 2011 Jan 19.
Published in final edited form as: Cancer Cell. 2010 Jan 19;17(1):53. doi: 10.1016/j.ccr.2009.11.021

Figure 1. IAP-mediated tumor cell invasion.

Figure 1

(A, B) Breast adenocarcinoma MDA-MB-231 (A) or prostate adenocarcinoma PC3 (B) cells transfected with control (Ctrl) or survivin (SVV)- or XIAP-directed siRNA, were analyzed by Western blotting after 48 h. *, non-specific. (C–E) The indicated siRNA transfected cell types were analyzed for invasion through Matrigel-coated Transwell inserts after 6 h by DAPI staining (C), and quantified (D). Scale bars, 200 μm (MDA-MB-231), 100 μm (PC3). (F) PC3 cells were transfected with control (Ctrl) or cIAP1-directed siRNA, and analyzed by PCR (left) and Matrigel invasion after 6 h (right). (G) MCF-7 or MCF-7 SVV cells (clones #1 and #2) were analyzed for Matrigel invasion after 6 h by DAPI staining (left), and quantified (right). Scale bars, 200 μm. For panels D–G data are the mean±SD of duplicates of a representative experiment out of at least two independent determinations. See also Figure S1.