Figure 5.

Incubation of fat cells with the nonfat cells does not significantly affect upregulation of IL-8, IL-1β or TNFα mRNA. The nonfat cells obtained by incubation of human adipose tissue with collagenase was incubated for 48 h either without or with the fat cells obtained from the same amount of tissue. The values are the mean ± SEM of the paired differences for 8 experiments from as many different individuals and shown as the ratio of mRNA in nonfat cells plus fat cells to that in nonfat cells. None of the differences were statistically significant with a P < 0.05.