Figure 3.
Overepression of A. fumigatus AfCrzA increased the mRNA accumulation of several genes. Fold increase in mRNA levels after the growth of the wild type and alcA::crzA mutant strain in MM+2% glycerol+2% ethanol for 6 hours at 37°C of (A) AfpmcB (Afu3g10690), (B) AfzfpA (Afu8g05010), (C) A. fumigatus Phospholipase D (Afu2g16520), (D) AfctfA (Afu4g03960), (E) Af BAR adaptor protein (Afu3g14230), (F) AfrcnA (Afu2g13060), (G) AfrfeF (Afu4g10200), (H) Af AAA ATPase (Afu4g04800), and (I) Afscf1 (Afu1g17370). The relative quantitation of all the genes and tubulin gene expression was determined by a standard curve (i.e., CT -values plotted against logarithm of the DNA copy number). The results are the means ± standard deviation of four sets of experiments. The values represent the number of times the genes are expressed compared to the corresponding wild type control strain (represented absolutely as 1.00).