Figure 2.

Metabolic remodeling is the primary effect of K_ATP channel deficiency. Proteins assigned by LTQ-Orbitrap MS/MS analysis from significantly altered spots were functionally categorized by Swiss-Prot ontological annotations. The primary association was with metabolic processes, as 63/102 protein assignments encompassed mitochondrial, cytoplasmic, and amino or nucleic acid metabolic functions. Protein names are listed with their Swiss-Prot gene name (for Node Symbol) to locate them in the protein interaction network, and with their corresponding spot numbers from their 2-D gel positions. Protein accession number, Mascot score, number of unique identified peptides, % sequence cov. (coverage), predicted M_r and pI for each protein (following expected post-translational processing, e.g., removal of a known or predicted mitochondrial signal peptide), and fold change (KO versus WT) are indicated. For proteins detected in more than one spot, maximum score and number of unique peptides are reported. Fold change was calculated as described in experimental procedures, and for proteins detected in both increasing and decreasing spots (**), both values are indicated. Complete MS/MS data for all proteins is outlined in Supplementary Table 1 (Supporting Information). (*TrEMBL entry; ** Contains both up- and down-regulated spots; † Node not detected for network analysis by Ingenuity Pathways; ‡ M_r/pI calculated after TargetP 1.1 prediction of mitochondrial localization and signal peptide cleavage site).