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Treatment of HIV-1 cell-associated virus. Chronically HIV-1-infected H9 (A) and PBMC (B) cells were incubated with serial two-fold dilutions of silver nanoparticles for 1 min and 60 min. Treated cells were centrifuged, washed three times with cell culture media, and then added to TZM-bl cells. Assessment of HIV-1 infection was made with a luciferase-based assay after 48 h. The assay was performed in triplicate; the error bars indicate the s.e.m.
