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Inhibition of p38 activity reverses the protection of L929 cells by cAMP from TNF-α-induced cell death. L929 cells were treated with or without SB203580 (SB, 1 μM, 30 min) prior to forskolin treatment (10 μM, 30 min), followed by stimulation with or without 10 ng/ml TNF-α for 24 h. Cell death was measured by PI staining. The percentages of cell death are shown in the right panel as mean ± SD; n = 3. Left panel is representative of three independent experiments.
