Figure 5.
The identification of SS1P/mesothelin complex in tumor ECF. TSKG2000 column was used to separate free SS1P and SS1P in complex. A, The overlapped chromatograms of standards, 20 μg of SS1P-biotin (dot line), 40 μg of mesothelin (dash line) and a mixture of 20 μg of SS1P-biotin and 25 μg of mesothelin (solid line). B, SS1P-biotin distribution in fractions of tumor ECF. Ten microgram of SS1P-biotin was injected. C, Mesothelin distribution in fractions of tumor ECF. Mice received 10 μg of SS1P-biotin. D, HA22-biotin distribution in fractions of tumor ECF. Twenty microgram of biotin-labeled HA22 was injected.