Table 5. List of primers used for semi-quantitative RT-PCR of cryptdine levels.
Primers | Sequence 5′ - 3′ |
Crypt1 forward | AAGAGACTAAAACTGAGGAGCAGC |
Crypt1 reverse | GGTGGTCATCAGGCACCAGCATCAGT |
Crypt4 forward | AAGAGACTAAAACTGAGGAGCAGC |
Crypt4 reverse | AGTGGTCATCAGGCCCCGGCATCAGC |
Crypt5 forward | AAGAGACTAAAACTGAGGAGCAGC |
Crypt5 reverse. | GGTGATCATCAGACCCCAGCATCAGT |
β actin forward | TGG AATCCT GTGGCATCCA |
β actin reverse | TAACAGTCCGCCTAGAAGCA |