Table 2.
Effect of polAexo and polBex on lac G·C→T·A mutant frequency in strains with impaired Pol III HE.a
| lac → lac+ (per 108) | |||
|---|---|---|---|
| Strain | polA+ | polAexo | polBex |
| mutL+ | 0.4 ± 0.2 | 1.3 ± 0.3 | 1.6 ± 1.0 |
| mutL+ dnaQ928 | 9.2 ± 2.2 | 11.6 ± 1.4 | 16 ± 7.0 |
| mutL+ dnaE486 | 2.8 ± 1.4 | 4.0 ± 1.6 | 251 ± 23 |
| mutL+ dnaE511 | 2.0 ± 1.4 | 3.9 ± 1.8 | 163 ± 35 |
| mutL | 1.4 ± 0.7 | 4.6 ± 2.3 | 8.4 ± 11 |
| mutL dnaQ49 | 44 ± 23 | 42 ± 12 | 120 ± 11 |
| mutL dnaN159 | 5.6 ± 1.7 | 9.8 ± 3.2 | 34 ± 7.0 |
| mutL dnaX36 | 56 ± 12 | 81 ± 16 | ND |
a
The experiments were performed with the lac G·C→T·A allele in the R(lagging) orientation. Mismatch repair deficient strains were mutL::Tn5. Mutant frequencies were determined as described in Experimental Procedures. Each entry is based on the median value for 10–20 independent cultures grown at 30°C (dnaQ49, dnaN159), 34°C (dnaQ928, dnaE486, dnaE511), or 37°C (dnaX36).
ND – not determined.