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. Author manuscript; available in PMC: 2010 Feb 9.
Published in final edited form as: Nature. 2009 Dec 10;462(7274):739. doi: 10.1038/nature08617

Figure 5. Human malignant gliomas containing R132 mutations in IDH1 contain increased concentrations of 2HG.

Figure 5

Human glioma samples obtained by surgical resection were snap frozen, genotyped to stratify as wild-type (WT) (N=10) or carrying an R132 mutant allele (Mutant) (n=12) and metabolites extracted for LC-MS analysis. Among the 12 mutant tumors, 10 carried a R132H mutation, one an R132S mutation, and one an R132G mutation. Each symbol represents the amount of the listed metabolite found in each tumor sample. Red lines indicate the group sample means. The difference in 2HG observed between WT and R132 mutant IDH1 mutant tumors was statistically significant by Student’s t-test (p<0.0001). There were no statistically significant differences in αKG, malate, fumarate, succinate, or isocitrate levels between the WT and R132 mutant IDH1 tumors.