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. Author manuscript; available in PMC: 2010 Aug 4.
Published in final edited form as: Nature. 2010 Feb 4;463(7281):627–631. doi: 10.1038/nature08728

Figure 2. Plasmepsin V cleaves PEXEL.

Figure 2

a, Plasmepsin V HA tagged (3D7-PMVHA). b, Plasmepsin V localises to ER. c, Inactive HA-tagged Plasmepsin V (3D7-PMVmutHA). d, Mutant Plasmepsin V (3D7-PMVmutHA) localises to ER. e, HA-tagged Plasmepsin V (ipPMVHA) with KAHRP peptides; unmutated, R>A, L>A, Q>A, RLQ>A. ipPMVmutHA (inactive) or ipPMIXHA (Plasmepsin IX) with unmutated peptide. f, Recovered enzyme. g, Recombinant GBP130 cleavage by Plasmepsin V. Last lanes are GBP130 mutant (RLE>A). U, uncleaved; C, cleaved. h, Identification of cleaved peptide GNGSGDSFDFRNKRTL. Identification of uncleaved RLQ>A mutant peptide. * refers to multiple H20 and NH3 losses. (see Supplementary for details)