Figure 1. Overview of CPTAC Unbiased Discovery Working Group interlaboratory studies.
All studies employed the NCI-20 reference mixture. Beginning with Study 3, a yeast lysate reference sample was introduced. Studies 2, 3, and 5 revealed ambiguities in the SOP that were corrected in subsequent versions. Studies 2–8 all prescribed blanks and washes between samples. Study 8 returned to lab-specific protocols in examining two different yeast concentrations. The full details of the SOP versions are available in Supplementary Information.