(A) Induction of inflammatory cytokine and chemokine mRNAs in lungs of MTS-exposed Jnk1−/− mice was analyzed as in Fig. 3B. Results are means ± S.E. (n = 5 for each group). Significant difference, *P < 0.05.
(B) Secretion of cytokines in lungs of MTS-exposed Jnk1−/− mice was analyzed as in Fig. 3C. Results are means ± S.E. (WT air control: n = 12; WT 4 cig./day: n = 9; Jnk1−/− air control: n = 9; Jnk1−/− 4 cig./day: n = 9). Significant difference, *P < 0.05.
(C) JNK1 deletion decreases MTS-induced lung cell proliferation. Results are means ± S.E. (WT air control: n = 7; WT 4 cig./day: n = 8; Jnk1−/− air control: n = 10; Jnk1−/− 4 cig./day: n = 9). Significant difference, *P < 0.0003.
(D) Lung appearance (left side) and histology (H&E stain; right side) in 5-month-old K-rasLA2 or K-rasLA2; Jnk1−/− mice with or without MTS exposure.
(E) Lung tumor multiplicity and maximal tumor sizes were determined as in Fig. 2D. Results are means ± S.E. (WT air control: n = 16; WT 4 cig./day: n = 18; Jnk1−/− air control: n = 13; Jnk1−/− 4 cig./day: n = 14). Significant difference, *P < 0.002. See also Figure S6.