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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Circ Res. 2009 Nov 19;106(2):346. doi: 10.1161/CIRCRESAHA.109.205724

Figure 4. Deregulated expression of major cardiac transcription factors in LAP2α-deficient mice.

Figure 4

A) MEF2c, GATA4 and their downstream targets, BNP and Myocardin A (MYOCA), are downregulated on mRNA level at specific stages in Lap2α−/− mice. B) STARS mRNA levels are lower only in old LAP2α-deficient hearts. C) Old Lap2α−/− mice exhibit decreased CTGF expression and normal TGFβ2 mRNA content. (Quantitative PCR (qPCR) and semiqPCR analyses of heart tissue. n = 4 – 5 male littermate pairs of each age. One outlier pair was excluded according to Grubb’s test. Samples were normalized for endogenous GAPDH levels in semiqPCR and HPRT (Hypoxanthine-Guanine Phosphoribosyltransferase) levels in qPCR and analyzed according to Pfaffl method44. Each knockout (KO) sample was compared to its respective WT littermate sample. Values are mean KO/WT expression ratios ± SE. *p<0.05, ANOVA).