Skip to main content
. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Mol Cell. 2010 Jan 15;37(1):143. doi: 10.1016/j.molcel.2009.12.018

Figure 4. CRL4Cdt2 E3 ubiquitin ligase complex promotes translesion DNA synthesis in unperturbed proliferating cells.

Figure 4

(A, B, and C) 293T cells were transfected with the indicated siRNA for twenty-four hours. UV-irradiated reporter plasmid was transfected into 293T cells. Replicated plasmids were recovered, Dpn1-digested and used to transform bacteria. Mutation frequency (see materials and methods) is expressed as the ratio of white to total (blue and white) colonies. Mean ± S.D. of 3 experiments (A and B) or 10 experiments (C). (D) Western blot of Cdt2 and Rad18 to show effectiveness of si-Cdt2 and si-Rad18 in 293T cells. (E) DT40 cells were transfected with si-Cdt2 for twenty-four hours and treated with cisplatin for seventy-two hours. Viable cells were counted and expressed relative to the control si-GL2 sample. Mean ± S.D. of 3 experiments.