Figure 2. Patterning of daughter bacteria in liquid without underneath substratum.

Bacterial divisions in LB medium were followed similar to that described in Figure 1. (A) Parental strain BW25113; (B) flagellum-null strain JW1923. In (A), arrowhead indicates a cell separating and disappearing from the bacterial string in the subsequent image. In (B), the arrow indicates bacteria that appeared as a string of four cells while the arrowhead marks a string of 16 offspring bacteria. Note: the balloon-type bacteria were not exactly on the focal plane of the microscope. No bacteria were observed to have a parallel or 4-cell array arrangement in all fields. Scale bar: 5 µm.