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. 2010 Feb 10;5(2):e9151. doi: 10.1371/journal.pone.0009151

Figure 4. SDS-PAGE and Western blot detection of purified recombinant MamK-like and MamK.

Figure 4

A) SDS-PAGE gel (10% acrylamide) with 2 µg of protein per lane stained with Coomassie blue. B) Western blot with 30 ng of protein per lane probed with anti-histidine tag antibody. C) Western blot with 30 ng of protein per lane probed with anti-MamK antibody. Molecular weights (MW) of protein standards are given on the left and apply to all panels. Expected MWs of MamK-like and MamK are 41 and 42 kDa respectively.