Skip to main content
. 2010 Feb 10;5(2):e9152. doi: 10.1371/journal.pone.0009152

Figure 1. Combined deficiency of Gab2 with heterozygous STAT5 reduces KLS cell responses to early acting cytokines.

Figure 1

KLS cells were sorted by flow cytometry and plated in 96-well plates in the presence of various cytokine cocktails. Total nucleated cells were counted 6 days later. IL-3 indicates murine IL-3 (20 ng/ml); 6, human IL-6 (50 ng/ml); S, murine stem cell factor (50 ng/ml); F, murine Flt3-ligand (50 ng/ml); and T, murine thrombopoietin (50 ng/ml). The cell expansion in each group of cytokines was plotted relative to wild-type control cells for each stimulation experiment. The results are the average ± SD for 3 experiments. Absolute cell expansions were comparable to previous descriptions[26], [28].