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. 2010 Feb 10;5(2):e9152. doi: 10.1371/journal.pone.0009152

Figure 3. Combined deficiency of Gab2 with heterozygous STAT5 allows replacement of newborn HSC with a donor graft.

Figure 3

A. Pups generated from inter-cross of Gab2+/−STAT5ab+/null mice were injected intraperitoneally with 1×107 CD45.1 positive BM cells 2–3 days after birth. At 4 weeks of age, the newly weaned mice were genotyped for STAT5 and Gab2. From multiple sets of experiments, totals of WT N = 22; STAT5ab+/null N = 27; Gab2−/− N = 9; and Gab2−/−STAT5ab+/null N = 5 mice were obtained and analyzed for evidence of long-term donor engraftment. B. At 16 weeks following injection, the mice were analyzed by flow cytometry of the peripheral blood leukocytes for engraftment with CD45.1 positive donor cells. Horizontal bars indicate the average for all mice analyzed per group. All comparisons were done by Mann-Whitney U test. C. BM was pooled from primary recipients and injected into 5 lethally-irradiated secondary recipients. Individual primary donors utilized for secondary transplantation are marked in panel B by open circles. The percentage of CD45.1 positive cells co-staining for Gr-1, B220, Ter119, or CD4 is shown from secondary transplantation and analysis 12 weeks later. The wild-type group was not included in the secondary transplantation since the primary recipients had little to no evidence of donor engraftment.