Figure 7. STAT5 and Gab2 double mutant fetal liver HSCs are defective in repopulation of secondary recipients.

At 14 weeks following transplant, 3 mice from each group were euthanized from the cohorts of Fig. 6 (Wild-type, Gab2^−/−, STAT5ab^null/null, and 3 sets of Gab2^−/−STAT5ab^null/null). BM cells were pooled from these primary recipient mice and transplanted into lethally-irradiated secondary CD45.1 hosts at a ratio of 1 donor to 5 recipient mice. A. Overall mouse survival was monitored on daily basis for WT N = 5; Gab2^−/− N = 4; STAT5ab^null/null N = 5; and Gab2^−/−STAT5ab^null/null N = 15. B. Donor engraftment in the peripheral blood of secondary transplanted mice for four combinations of STAT5 and Gab2 genotype that survived past approximately 12 weeks for WT N = 5; Gab2^−/− N = 4; STAT5ab^null/null N = 5; and Gab2^−/−STAT5ab^null/null N = 4. C. The absolute number of Gr-1 positive cells was determined by antibody staining and flow cytometry combined with the total white blood cell count per µl of whole blood. Horizontal bars indicate the average for all mice analyzed per group.