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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: J Proteome Res. 2010 Feb 5;9(2):945. doi: 10.1021/pr9008207

Figure 2.

Figure 2

Phosphorylation of S6 Ser 235/236 in bleomycin-treated HeLa cells overexpressing PP5 (H304Q) or WT-PP5. HeLa cells expressing control or induced WT-PP5 or PP5(H304Q) were treated with bleomycin, then lysates prepared and subjected to western blot analysis for phospho-Ser 235/236 S6 or total S6. Cadherin was monitored as the loading control. Blots were quantified as described in Material and Methods. The log base 2 value of the difference of control PP5(H304Q) response – WT-PP5 response was 1.842 and for overexpressed PP5(H304Q) – WT-PP5 was 0.580 in the blots shown. Western blot analyses performed for 2 independent biological replicates yielded similar results.

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