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YB-1 levels in bleomycin-treated cells overexpressing PP5 (H304Q) relative to cells overexpressing WT-PP5. HeLa cells expressing control or induced WT-PP5 or PP5(H304Q) were treated with bleomycin, then lysates prepared and subjected to western blot analysis for YB-1. GAPDH was monitored as the loading control. Western blot analyses were performed twice, with similar results.
