Table 2.
Overlap of differentially expressed phosphoproteins between biological replicates (BR) 1 and 2, with similar but not identical peptides
| Reference (IPI) |
Peptide | BR 1 Cntl1 |
BR 1 OE1 |
BR 2 Cntl1 |
BR2 OE1 |
Protein | Residue (previously identified) |
Protein involved in DDR |
Regulation |
|---|---|---|---|---|---|---|---|---|---|
| 00099730 |
2[SGT]PPRQGSIT*SPQANEQ[ SVT]PQRR |
1.24 | −1.16 | Splicing Coactivator SRm300 |
S846 5, T848 5, T856 (No), T866 5, 60 |
Yes 44 | Down | ||
| [SGT]PPRQGSITSPQANEQ[S VT]PQRR |
1.25 | 1.10 | Down | ||||||
| 00021840 | L[SS]LRAS*TSKSESSQK | 2.35 | 0.52 | 40S ribosomal protein S6 | S235 2, 59, 62–64, | Yes [41–43] | Down | ||
| RL[SS]LRAS*TSKSESSQK | 1.33 | −0.91 | S236 2, 59, 62–64, S240 43, 59, | Down | |||||
| L[SS]LRAS*TSKS*ESSQK | 1.36 | 0.77 | 62, 64–66, S244 43, 59, 64–66 | Down | |||||
| RLS*S*LRAS*TSKSESSQK | −2.06 | −5.19 | Down | ||||||
| RLS*S*LRASTSK | −1.35 | −3.20 | Down | ||||||
| RLS*S*LRASTSKSESSQK | −0.53 | −2.87 | Down | ||||||
| 00008274 | SGPKPFS*APKPQTSPS*PK | −1.61 | −2.28 | Adenylyl cyclase- | S310 (No) | No | Down | ||
| SGPKPFSAPKPQT*SPSPK | −0.97 | 0.95 | associated protein 1 | T307 (No) | Up |
1
The values in each of the following columns- BR1 Cntl, BR1 OE, BR2 Cntl, BR2 OE, represent the difference of the (log base 2) average response from PP5(H304Q) and (WT-PP5) (i.e. average PP5(H304Q) response – average (WT-PP5) response). If the average difference (within each biological replicate) going from the control (Cntl) to the overexpressed (OE) condition increases then the regulation is up, if the average difference from the control to the OE condition decreases then the regulation is down. DDR, DNA damage response
2
Residues placed within brackets represent ambiguity in phosphorylation site assignment.