Skip to main content
. 2009 Dec 22;107(2):906–911. doi: 10.1073/pnas.0913415107

Fig. 1.

Fig. 1.

PLC-γ1 contributes to the high NaCl–induced increase in TonEBP/OREBP transcriptional activity. After transfection, osmolality either was kept at 300 mosmol/kg or was increased to 500 mosmol/kg by adding NaCl for 16 h before measuring reporter activity. (A) TonEBP/OREBP transcriptional activity in MEFs deficient in PLC-γ1 (Null) and the same cells reconstituted with PLC-γ1 (Plus). Luciferase reporters are ORE-X and an otherwise identical reporter that does not contain a TonEBP/OREBP binding site, “Promoter.” (B) Effect of siRNA knockdown of PLC-γ1 on TonEBP/OREBP transcriptional activity (ORE-X reporter) in cells transfected with control (nontargeting) or PLC-γ1-specific siRNA for 48 h. Inset: Effect of the siRNAs on PLC-γ1 protein expression. (C) Stimulation of TonEBP/OREBP by PLC-γ1 is dependent on its lipase activity. Effect of conditional (Tet-on) over expression of wild-type PLC-γ1 and dominant negative LIM PLC-γ1 in HEK293 cells. Inset: Expression of the Flag-PLC-γ1 constructs. (D and E) Effect of the phospholipase inhibitor U73122. U73122 or vehicle (DMSO) control was added from 30 min before changing osmolality. Mean ± SEM. *P ≤ 0.05; n = 3.