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Effect of amino acid replacements on the catalytic activity of HOD and QDO
| KM, QND |
 , QND |
 *
|
|
| HOD | 2.7 | 38.4 | 100 |
| HOD H251A | 59.0 | 0.0034 | 0.0004 |
| HOD D126A | 27.2 | 1.05 | 0.27 |
| HOD S101A | 162.0 | 46.4 | 2.01 |
| HOD H38A | 159.5 | 3.0 | 0.13 |
| HOD H102L | 23.3 | 0.027 | 0.008 |
| KM, QNL |
 , QNL |
 *
|
|
| QDO | 10.4 | 20.6 | 100 |
| QDO D120A | 180.9 | 2.55 | 0.71 |
The units for KM are μM, and 
are in s-1.
*Apparent kcat/Km relative to the wild-type enzyme expressed as percentage.
