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Immunodot analysis of pectin in PG and PMEI plants. ChASS fractions were extracted from leaves of untransformed and transgenic Arabidopsis PG (A) and PMEI plants (B) and of untransformed and transgenic wheat AcPMEI plants (C). Numbers indicate different independent transgenic lines. The indicated amounts (in μg) of ChASS fraction were applied at a single point to a nitrocellulose membrane. Specific HGA epitopes were detected by using PAM1 and JIM5 monoclonal antibodies.
