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Conformational analysis of triple-helical CRPs. (A) CD spectra at 4 °C of solutions of CRPs (200 μM in 50 mM HOAc) after their incubation at ≤ 4 °C for ≥24 h. (B) Effect of temperature on the molar ellipticity (224, 225, or 226 nm) of the solutions of A. Data were recorded at 3 °C intervals after a 5 min. equilibration. Two-state fits are displayed as lines.
