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. 2009 Dec 22;107(2):850–855. doi: 10.1073/pnas.0911948107

Fig. 1.

Fig. 1.

The majority of infected B cells express EBV immediate-early genes. (A) Western blot immunodetection of Zta in primary B cells infected with the B95.8 strain of EBV at different time points after infection. HEK293 cells transiently transfected with a BZLF1 expression plasmid serve as positive control. β-actin signals served as loading control. (B) By semiquantitative RT-PCR analysis, B95.8 EBV-infected B cells detectably express the immediate-early genes BZLF1 (a fully spliced and an unspliced transcript) and BRLF1 and the early gene BMRF1 but do not express the late genes BALF4 and BLLF1 essential for virus synthesis during the first 11 days of infection. (C) Construction of the reporter EBV with the rat CD2 gene expressed from the viral BMRF1 promoter. Shown are parts of the wild-type (2089) and reporter (3875) EBV genomes and their functionally relevant genes (26). (D) The majority of GFP-positive B cells infected with the BMRF1 reporter EBV strain express rat CD2 very early after infection.