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Binding of mAb F77 to prostate cancer cell lines. Androgen-dependent LNCaP and androgen-independent Du145 and PC3 are commonly used prostate cell lines. PC3-MM2 is a highly metastatic PC3-derived cell line. RWPE-1 is a normal human prostate epithelial cell line immortalized by HPV-18. Tumorigenic prostate epithelial cell line RWPE-2 was derived from RWPE-1 with K-ras oncogene transfection. mAb F77 is represented by the bold line and irrelevant mouse IgG3 by the dashed line.
